The financial analysis has been worked out considering 100% of the capital expenditure on the establishment of the laboratory and 50% of the first year's operational cost except the rent of the building which is considered for 12 months. The financial outlay and bank loan works out as follows:
Financial Outlay Rs : 9.360 lakh
Margin Money ( 25% ) Rs : 2.340 lakh
Bank Loan Rs : 7.020 lakh
Interest rate for ultimate borrowers
Banks are free to decide the rate of interest within the overall RBI guidelines. However, for working out the financial viability and bankability of the model project we have assumed the rate of interest as 12% per annum.
Interest rate for refinance from NABARD
As per the circulars of NABARD issued from time to time
The financial viability of the unit is based on the assumption that a minimum of 1000 samples would be tested both for PCR test and water analysis and 500 samples for soil analysis each year, which has been shown in annexure -V. The financial parameters are indicated below :
a) NPW @ 15% DF = Rs.4.495 lakh
b) BCR @ 15% DF = 1.2 : 1
c) IRR 58 %
The repayment of loan is possible in six years without any grace period as income generation starts in the first year itself as shown in annexure – V.
Banks may take a decision as per RBI guidelines.
Annexure – I
Technical Parameters for PCR test, Soil and Water Analysis
1. Technical Parameters for PCR test
The process of diagnosing diseases in the shrimp seeds uses a process known as Polymerase Chain Reaction ( PCR ). The PCR test accurately identifies virus-infected seed. The process involves
(i) Collection of samples, preservation and processing for histopathology and PCR amplification. (ii) Total Nucleic acid extraction from samples collected.
(iii) Extraction of DNA and RNA from samples obtained from suspected shrimp and visualization of the extracted fraction through agarose gel electophoresis.
A sample is taken from about 75 to 150 shrimp seeds and digestion buffer is added to it. The sample is crushed and put into micro lids and incubated for 30 minutes at room temperature. It is then centrifuged at 5000 rpm for 5 minutes and the supernatant of 500 micro liters is taken to which the precipitation buffer of 500 micro liters is added. The solution is centrifuged for 20 minutes at 14000 rpm. The supernatant is discarded and the residue is taken to which 500 micro liters of washing buffer is added. This solution is centrifuged for 10 minutes at 14000 rpm. Again the supernatant is discarded and the remains are dried on laminar flow for 5 minutes. To this 150 liters of distilled water is added to dissolve it uniformly. Primers are added to positive control, negative control and samples. The samples are kept in thermocyler after which, the liquid is poured into the wells on a gel in electrophoretic unit. The bands are then compared under UV trans illuminator for detection of the viruses. The process has been indicated in a flow chart in annexure – II.
2. Soil and Water Analysis
There are number of parameters that need to be analyzed before establishing a farm. During the farming also, regular monitoring of the water and soil parameters is necessary in order to take the full benefit of the nutrients available in the soil as also in the water. Important parameters that need to be evaluated in the soil and water are
- Dissolved Oxygen
- Water Retention etc.
Different tests are required to be done to analyze the above parameters, based on which necessary action can be initiated to provide correct dose of fertilizers and supplementary feed. It may not be possible for every farmer to have facility for undertaking all these tests, therefore such facility which was hitherto available through State Departments free of cost can be provided through these laboratories.